Microdevice DNA forensics by the simple tandem repeat method.

Nils Goedecke; Brian McKenna; Sameh El-Difrawy; Elizabeth Gismondi; Abigail Swenson; Loucinda Carey; Paul Matsudaira; Daniel J Ehrlich

doi:10.1016/j.chroma.2005.05.100

PubMed · 16569579

Microdevice DNA forensics by the simple tandem repeat method.

Nils Goedecke·Brian McKenna·Sameh El-Difrawy·Elizabeth Gismondi·Abigail Swenson·Loucinda Carey·Paul Matsudaira·Daniel J Ehrlich

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Abstract

We review recent experiments on DNA forensics by the simple tandem repeat (STR) method using a 16-lane micromachined device as the active separation element. Separations by linear polyacrylamide matrices show very high data quality metrics when evaluated with statistically significant data sets. Full 16-locus multiplexes are verified on the multilane system. Multi-donor mixed samples are studied in the context of the limits of the laser-induced fluorescence detector and data-reduction software. The microdevice appears to be posed to outperform current capillary arrays in terms of stability and, through specialized sample loading, in the interpretation of complex mixtures.

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Nils Goedecke, Brian McKenna, Sameh El-Difrawy, Elizabeth Gismondi, Abigail Swenson, Loucinda Carey, Paul Matsudaira, Daniel J Ehrlich. 2005-06-22. Microdevice DNA forensics by the simple tandem repeat method.. https://doi.org/10.1016/j.chroma.2005.05.100

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