Fluorescence quencher improves SCANSYSTEM for rapid bacterial detection.

M Schmidt; M K Hourfar; A Wahl; S-B Nicol; T Montag; W K Roth; E Seifried

doi:10.1111/j.1423-0410.2006.00763.x

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PubMed · 16635069

Fluorescence quencher improves SCANSYSTEM for rapid bacterial detection.

M Schmidt·M K Hourfar·A Wahl·S-B Nicol·T Montag·W K Roth·E Seifried

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Abstract

BACKGROUND AND OBJECTIVES: The optimized scansystem could detect contaminated platelet products within 24 h. However, the system's sensitivity was reduced by a high fluorescence background even in sterile samples, which led to the necessity of a well-trained staff for confirmation of microscope results. MATERIALS AND METHODS: A new protocol of the optimized scansystem with the addition of a fluorescence quencher was evaluated. Pool platelet concentrates contaminated with five transfusion-relevant bacterial strains were tested in a blind study. RESULTS: In conjunction with new analysis software, the new quenching dye was able to reduce significantly unspecific background fluorescence. Sensitivity was best for Bacillus cereus and Escherichia coli (3 CFU/ml). DISCUSSION: The application of a fluorescence quencher enables automated discrimination of positive and negative test results in 60% of all analysed samples.

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Bacteriological Techniques Blood Platelets Colony Count, Microbial Fluorescent Dyes Humans Sensitivity and Specificity Sepsis Transfusion Reaction Journal Article

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M Schmidt, M K Hourfar, A Wahl, S-B Nicol, T Montag, W K Roth, E Seifried. 2006. Fluorescence quencher improves SCANSYSTEM for rapid bacterial detection.. https://doi.org/10.1111/j.1423-0410.2006.00763.x

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