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PubMed · 16679564

Single-step multicolor fluorescence in situ hybridization using semiconductor quantum dot-DNA conjugates.

Laurent A Bentolila·Shimon Weiss
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Abstract

We report a rapid method for the direct multicolor imaging of multiple subnuclear genetic sequences using novel quantum dot-based fluorescence in situ hybridization (FISH) probes (QD-FISH). Short DNA oligonucleotides were attached on QDs and used in a single hybridization/detection step of target sites in situ. QD-FISH probes penetrate both intact interphase nuclei and metaphase chromosomes and showed good targeting of dense chromatin domains with minimal steric hindrances. We further demonstrated that QD's broad absorption spectra allowed different colored probes specific for distinct subnuclear genetic sequences to be simultaneously excited with a single excitation wavelength and imaged free of chromatic aberrations in a single exposure. Thus, these results demonstrate that QD-FISH probes are very effective in multicolor FISH applications. This work also documents new possibilities of using QD-FISH probes detection down to the single molecule level.

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AnimalsBiotinylationCell Culture TechniquesCell LineCentromereColorDNAHeLa CellsHeterochromatinHumansIn Situ Hybridization, FluorescenceMast CellsMiceMicroscopy, ConfocalModels, ChemicalNucleic Acid HybridizationOligonucleotidesProtein Structure, TertiaryQuantum DotsJournal ArticleResearch Support, N.I.H., Extramural

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Laurent A Bentolila, Shimon Weiss. 2006. Single-step multicolor fluorescence in situ hybridization using semiconductor quantum dot-DNA conjugates.. https://doi.org/10.1385/cbb%3A45%3A1%3A59

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