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Controlling bone morphogenetic protein diffusion and bone morphogenetic protein-stimulated bone growth using fibrin glue.

Vikas V Patel·Li Zhao·Pamela Wong·Linda Kanim·Hyun W Bae·Ben B Pradhan·Rick B Delamarter
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Abstract

STUDY DESIGN: An in vitro and in vivo study. OBJECTIVE: To evaluate the ability of fibrin glue to limit diffusion of recombinant human bone morphogenetic protein (rhBMP)-2 and its ability to protect spinal nerves from rhBMP-2 stimulated bone growth. SUMMARY OF BACKGROUND DATA: Studies have shown bone morphogenetic protein (rhBMP-2) stimulated bone growth can encroach on the spinal canal and nerves, causing neural compression. More recently, rhBMP-2 use in the cervical spine has been associated with life-threatening swelling. Fibrin glue has been used as a biologic carrier but has not been evaluated for its ability to limit rhBMP-2. METHODS: In phase 1 of the study, rhBMP-2 soaked absorbable collagen sponges (ACS) were encapsulated in fibrin glue and immediately incubated in physiologic lactated ringers solution at 38 degrees C. Samples of solution were tested for rhBMP-2 concentration. In phase 2 of the study, rats were surgically treated with laminectomy and placement of rhBMP-2/ACS versus laminectomy and placement of fibrin glue before placement of rhBMP-2/ACS. After 8 weeks, animals were euthanized and imaged using micro-computerized tomography. RESULTS: The diffusion study showed a significant limitation in rhBMP-2 diffusion when encapsulated in fibrin glue. The laminectomy study revealed blockage of bone formation by fibrin glue and protection of the spinal canal. CONCLUSIONS: Fibrin glue can limit the diffusion of rhBMP-2, and, thus, it can be used to help protect the spinal canal and nerve roots from rhBMP-2 stimulated bone growth.

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AnimalsBone Morphogenetic Protein 2Bone Morphogenetic ProteinsDiffusionFemaleFibrin Tissue AdhesiveGelatin Sponge, AbsorbableHumansLaminectomyLumbar VertebraeRatsRats, Inbred LewRecombinant ProteinsSpinal CanalTransforming Growth Factor betaComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Vikas V Patel, Li Zhao, Pamela Wong, Linda Kanim, Hyun W Bae, Ben B Pradhan, Rick B Delamarter. 2006-05-15. Controlling bone morphogenetic protein diffusion and bone morphogenetic protein-stimulated bone growth using fibrin glue.. https://doi.org/10.1097/01.brs.0000217650.90861.99

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