Generation of equally sized particle plaques using solid-liquid suspensions.

Tim Herrmann; Magnus Schröder; Jürgen Hubbuch

doi:10.1021/bp050296i

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PubMed · 16739980

Generation of equally sized particle plaques using solid-liquid suspensions.

Tim Herrmann·Magnus Schröder·Jürgen Hubbuch

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Abstract

A device is presented for the generation of equally sized plaques of sensitive particles in a 96-well format. The resulting particle plaques can be used for the measurement of adsorption isotherms and uptake kinetics in protein chromatography or for immobilization reactions. The particle plaques are formed from suspensions with a vacuum device that is designed as a reusable sandwich module. The particles are retained by a mesh while the solvent is removed by the vacuum. As most particles used for protein chromatography are sensitive to mechanical stress and dehydration, the vacuum device is gentle enough to allow the use of these particles, thus eliminating the uncertainty of slurry preparation and pipetting. Apparatus characteristics and preparation procedures are described precisely. The physical intactness of the particles after the preparation procedure is proved by microscopic analysis. Data on the uniformity of the obtained resin plaques with respect to the reproducibility of their adsorption performance is given. Finally, adsorption isothermal and kinetic data of BSA on an ordinary HIC system obtained by high-throughput measurements are shown as an application example.

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Adsorption Chromatography, Liquid Equipment Design Immobilization Kinetics Particle Size Proteins Solvents Thermodynamics Journal Article Research Support, Non-U.S. Gov't

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Tim Herrmann, Magnus Schröder, Jürgen Hubbuch. Generation of equally sized particle plaques using solid-liquid suspensions.. https://doi.org/10.1021/bp050296i

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