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Protamine-precipitated estrogen receptor: a solid-phase ligand exchange assay.

G C Chamness·K Huff·W L McGuire
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Abstract

Cytoplasmic estrogen receptor can exist either free (R) or bound to estradiol-17beta (RE). Both forms can be precipitated from cytosols by protamine sulfate. After protamine precipitation R binds 3-H-estradiol-17beta quantitatively at either 0 degrees or 30 degrees, while precipitated RE binds 3-H-estradiol-17beta only at 30 degrees by exchanging with previously bound hormone. Using these observations, we have developed a method for separate determination of both cytoplasmic R and RE. This method should also be applicable for assay of other steroid receptors, especially in cases where interfering components are present in the whole cytosol.

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AnimalsCastrationCharcoalChemical PrecipitationCytoplasmDextransEstradiolFemaleKineticsLigandsMethodsOvaryProtaminesRatsReceptors, Cell SurfaceTemperatureTritiumUterusBiologyEndocrine SystemEstradiol--analysisEstrogensExaminations And DiagnosesHormone ReceptorsHormonesLaboratory Examinations And DiagnosesLaboratory ProceduresMembrane ProteinsPhysiologyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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G C Chamness, K Huff, W L McGuire. 1975. Protamine-precipitated estrogen receptor: a solid-phase ligand exchange assay.. https://doi.org/10.1016/0039-128x(75)90017-3

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