Solution-phase molecular-scale computation with deoxyribozyme-based logic gates and fluorescent readouts.

Joanne Macdonald; Darko Stefanovic; Milan N Stojanovic

doi:10.1385/1-59745-069-3:343

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Solution-phase molecular-scale computation with deoxyribozyme-based logic gates and fluorescent readouts.

Joanne Macdonald·Darko Stefanovic·Milan N Stojanovic

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Abstract

Recent development of solution-phase molecular-scale Boolean calculations using deoxyribozymes is potentially an important step toward the development of autonomous therapeutic and diagnostic devices. Here, the construction of basic YES, AND, ANDNOT, and ANDANDNOT deoxyribozyme-based logic gates is described. Protocols for testing gate activity using fluorescent oligonucleotide probes have been provided, and pointers for gate optimization are included.

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Base Sequence Computers, Molecular DNA, Catalytic Fluorescence Resonance Energy Transfer Fluorescent Dyes Molecular Probes Molecular Sequence Data Oligonucleotides Rhodamines Journal Article Review

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Joanne Macdonald, Darko Stefanovic, Milan N Stojanovic. 2006. Solution-phase molecular-scale computation with deoxyribozyme-based logic gates and fluorescent readouts.. https://doi.org/10.1385/1-59745-069-3%3A343

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