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PubMed · 16793611

[Cloning of A73 gene and its coding sequence analysis].

Ang Li·Song Zhao·Wei Hu·Gao-feng Zhao·Xiao-shi Zhang
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Abstract

OBJECTIVE: To clone A73 gene of Epstein-Barr virus (EBV) and examine the variation of its coding sequence. METHODS: A73 coding sequence (CDS) amplified from 7 patients in nasophryngeal carcinoma (NPC) biopsies by RT-PCR was cloned into pGEM-T-Easy vector to construct the recombinant plasmid, which was subjected to sequence analysis in Gen Bank database using Blast software. RESULTS: A73 gene from nasophryngeal carcinoma was successfully cloned into pGEM-T Easy vector. A locus with conversion of A-->C in A73 was found at CDS 45 nt, located in the exonVB157154 nt, which did not result in an amino acid replacement, and the variation frequency was 7/7. CONCLUSION: There is a point mutation in A73 CDS of EBV isolated from NPC tissue, which might produce NPC-associated polymorphism with possible involvement in the composition of some subtype of EB virus.

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Cloning, MolecularDNA, ComplementaryHerpesvirus 4, HumanHumansMolecular Sequence DataNasopharyngeal NeoplasmsOpen Reading FramesPoint MutationSequence Analysis, DNAViral ProteinsEnglish AbstractJournal Article

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Ang Li, Song Zhao, Wei Hu, Gao-feng Zhao, Xiao-shi Zhang. 2006. [Cloning of A73 gene and its coding sequence analysis].. https://pubmed.ncbi.nlm.nih.gov/16793611/

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