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Ordering three DNA polymorphisms on human chromosome 3 by sperm typing.

T M Goradia·V P Stanton·X F Cui·H Aburatani·H H Li·K Lange·D E Housman·N Arnheim
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Abstract

Three loci on the short arm of human chromosome 3 were ordered by sperm typing to expand the limited genetic map of this region. Almost 300 individual sperm from a donor triply heterozygous at D3S2, D3S11, and D3S12 were amplified by PCR using primers flanking the polymorphic site at each locus. Primary PCR product was reamplified using allele-specific primers of different lengths, allowing the allelic state at each locus to be determined by gel electrophoresis. Maximum likelihood analysis of the sperm-typing data showed that the most likely order was D3S2-D3S11-D3S12 with an odds ratio of almost 5000:1 when compared to the next most likely order. This finding should be useful in interpreting loss of heterozygosity on 3p in a variety of cancers. Our results also demonstrate the practicality of ordering DNA polymorphisms using sperm typing.

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AllelesBase SequenceChromosome MappingChromosomes, Human, Pair 3DNAGenetic MarkersHeterozygoteHumansMaleModels, GeneticMolecular Sequence DataPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthSpermatozoaJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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T M Goradia, V P Stanton, X F Cui, H Aburatani, H H Li, K Lange, D E Housman, N Arnheim. 1991. Ordering three DNA polymorphisms on human chromosome 3 by sperm typing.. https://doi.org/10.1016/0888-7543(91)90459-r

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