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A simple, rapid and effective method for total RNA extraction from Lentinula edodes.

Jun-Hui Li·Chuan-Hong Tang·Chun-Yan Song·Ming-Jie Chen·Zhi-Yong Feng·Ying-Jie Pan
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Abstract

A rapid, inexpensive and reliable method for total RNA extraction from fruiting bodies of Lentinula edodes containing large quantities of polysaccharides and secondary metabolites is described. An initial extraction step using saturated NaCl solution facilitates the separation of nucleic acids from contaminants and, after further extraction with organic solvents and precipitation with 2-propanol, total RNA of high purity and suitable for applications such as cDNA synthesis, RT-PCR and Northern blot hybridization was obtained. The procedure may also have wider applicability for total RNA extraction from the tissues of other mushrooms.

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Blotting, NorthernFruiting Bodies, FungalRNASaline Solution, HypertonicShiitake MushroomsJournal Article

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Jun-Hui Li, Chuan-Hong Tang, Chun-Yan Song, Ming-Jie Chen, Zhi-Yong Feng, Ying-Jie Pan. 2006-06-24. A simple, rapid and effective method for total RNA extraction from Lentinula edodes.. https://doi.org/10.1007/s10529-006-9074-y

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