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PubMed · 16829567

Lactosylceramide: lateral interactions with cholesterol.

Xiuhong Zhai·Xin-Min Li·Maureen M Momsen·Howard L Brockman·Rhoderick E Brown
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Abstract

Lactosylceramide (LacCer) is a key intermediate in glycosphingolipid metabolism and is highly enriched in detergent-resistant biomembrane fractions associated with microdomains, i.e., rafts and caveolae. Here, the lateral interactions of cholesterol with LacCers containing various homogeneous saturated (8:0, 16:0, 18:0, 24:0) or monounsaturated acyl chains (18:1, 24:1) have been characterized using a Langmuir-type film balance. Cholesterol-induced changes in lateral packing were assessed by measuring changes in average molecular area, i.e., area condensations, and in lateral elasticity, i.e., surface compressional moduli (C S(-1)) with emphasis on high surface pressures (> or = 30 mN/m) that mimic biomembrane conditions. Cholesterol most dramatically affected the lateral packing elasticity of LacCers with long saturated acyl chains at sterol mole fractions > or = 0.3, consistent with liquid-ordered (LO) phase formation. The lateral elasticity within the LacCer-cholesterol LO-phase was much lower than that observed within pure LacCer condensed, i.e., gel, phase. The magnitude of the cholesterol-induced reduction in lateral elasticity was strongly mitigated by cis monounsaturation in the LacCer acyl chain. At identical high sterol mole fractions, higher lateral elasticity was observed within LacCer-cholesterol mixtures compared with galactosylceramide-cholesterol and sphingomyelin-cholesterol mixtures. The results show how changes to sphingolipid headgroup and acyl chain structure contribute to the modulation of lateral packing elasticity in sphingolipid-cholesterol LO-phases.

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Antigens, CDCholesterolElasticityLactosylceramidesLipid BilayersPhase TransitionSphingomyelinsJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't

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Xiuhong Zhai, Xin-Min Li, Maureen M Momsen, Howard L Brockman, Rhoderick E Brown. 2006-07-07. Lactosylceramide: lateral interactions with cholesterol.. https://doi.org/10.1529/biophysj.106.084921

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