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Numerical model for DNA loading in microdevices: stacking and autogating effects.

Sameh A El-Difrawy·Alok Srivastava·Elizabeth A Gismondi·Brian K McKenna·Daniel J Ehrlich
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Abstract

Many electrophoresis-based DNA sequencing and genotyping microdevices rely on field-driven effects to load and preconcentrate the sample. A quantitative model is developed for a broad class of electrophoresis-based microfabricated sample injectors. Quantitative predictions of DNA preconcentration are compared with experimental data and are shown to qualitatively reproduce the detailed time-evolving sample distribution in the injector. The model provides practical guidance on device and protocol design, in order to optimize this critical aspect of microfluidic devices.

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DNAElectrophoresis, CapillaryElectrophoresis, MicrochipFlow Injection AnalysisModels, ChemicalSequence Analysis, DNAJournal Article

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Sameh A El-Difrawy, Alok Srivastava, Elizabeth A Gismondi, Brian K McKenna, Daniel J Ehrlich. 2006. Numerical model for DNA loading in microdevices: stacking and autogating effects.. https://doi.org/10.1002/elps.200600088

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