Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system.

Raluca-Ioana Stefan-van Staden; Rahel Girmai Bokretsion; Jacobus F van Staden; Hassan Y Aboul-Enein

doi:10.1080/10826060600912393

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Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system.

Raluca-Ioana Stefan-van Staden·Rahel Girmai Bokretsion·Jacobus F van Staden·Hassan Y Aboul-Enein

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Abstract

Carbon paste based biosensors for the determination of creatine and creatinine have been integrated into a sequential injection system. Applying the multi-enzyme sequence of creatininase (CA), and/or creatinase (CI) and sarcosine oxidase (SO), hydrogen peroxide has been detected amperometrically. The linear concentration ranges are of pmol/L to nmol/L magnitude, with very low limits of detection. The proposed SIA system can be utilized reliably for the on-line simultaneous detection of creatine and creatinine in pharmaceutical products, as well as in serum samples, with a rate of 34 samples per hour and RSD values better than 0.16% (n=10).

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Amidohydrolases Biosensing Techniques Carbon Creatine Creatinine Enzymes, Immobilized Flow Injection Analysis Hydrogen Peroxide Online Systems Sarcosine Oxidase Ureohydrolases Journal Article

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Raluca-Ioana Stefan-van Staden, Rahel Girmai Bokretsion, Jacobus F van Staden, Hassan Y Aboul-Enein. 2006. Simultaneous detection of creatine and creatinine using a sequential injection analysis/biosensor system.. https://doi.org/10.1080/10826060600912393

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