Cork Oak Trees (Quercus suber L.).

Rubén Alvarez; Mariano Toribio; Millán Cortizo; Ricardo-Javier Ordás Fernández

doi:10.1385/1-59745-131-2:113

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PubMed · 17033056

Cork Oak Trees (Quercus suber L.).

Rubén Alvarez·Mariano Toribio·Millán Cortizo·Ricardo-Javier Ordás Fernández

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Abstract

A transformation system for selected mature Quercus suber L. trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries the nptII and uidA genes. Evidence of stable transgene integration is obtained by polymerase chain reaction for nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos are germinated and successfully transferred to soil.

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Agrobacterium tumefaciens Blotting, Southern Cell Culture Techniques Coculture Techniques Culture Media Germination Glucuronidase Plant Leaves Plants, Genetically Modified Plasmids Polymerase Chain Reaction Quercus Seeds Soil Transformation, Genetic Transgenes Journal Article Research Support, Non-U.S. Gov't

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Rubén Alvarez, Mariano Toribio, Millán Cortizo, Ricardo-Javier Ordás Fernández. 2006. Cork Oak Trees (Quercus suber L.).. https://doi.org/10.1385/1-59745-131-2%3A113

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