Double-labelled HIV-1 particles for study of virus-cell interaction.

Marko Lampe; John A G Briggs; Thomas Endress; Bärbel Glass; Stefan Riegelsberger; Hans-Georg Kräusslich; Don C Lamb; Christoph Bräuchle; Barbara Müller

doi:10.1016/j.virol.2006.10.005

PubMed · 17097708

Double-labelled HIV-1 particles for study of virus-cell interaction.

Marko Lampe·John A G Briggs·Thomas Endress·Bärbel Glass·Stefan Riegelsberger·Hans-Georg Kräusslich·Don C Lamb·Christoph Bräuchle·Barbara Müller

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Abstract

Human immunodeficiency virus (HIV) delivers its genome to a host cell through fusion of the viral envelope with a cellular membrane. While the viral and cellular proteins involved in entry have been analyzed in detail, the dynamics of virus-cell fusion are largely unknown. Single virus tracing (SVT) provides the unique opportunity to visualize viral particles in real time allowing direct observation of the dynamics of this stochastic process. For this purpose, we developed a double-coloured HIV derivative carrying a green fluorescent label attached to the viral matrix protein combined with a red label fused to the viral Vpr protein designed to distinguish between complete virions and subviral particles lacking MA after membrane fusion. We present here a detailed characterization of this novel tool together with exemplary live cell imaging studies, demonstrating its suitability for real-time analyses of HIV-cell interaction.

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Biological Transport Cell Line Cell Membrane Cell Nucleus Gene Products, vpr Green Fluorescent Proteins HIV Infections HIV-1 Humans Luminescent Proteins Staining and Labeling Viral Matrix Proteins Virology Virus Replication vpr Gene Products, Human Immunodeficiency Virus Red Fluorescent Protein Journal Article Research Support, Non-U.S. Gov't

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Marko Lampe, John A G Briggs, Thomas Endress, Bärbel Glass, Stefan Riegelsberger, Hans-Georg Kräusslich, Don C Lamb, Christoph Bräuchle, Barbara Müller. 2006-11-09. Double-labelled HIV-1 particles for study of virus-cell interaction.. https://doi.org/10.1016/j.virol.2006.10.005

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