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PubMed · 17162738

Comparing transfection efficiency and safety for antisense oligodeoxyribonucleotide between phospholipids-based microbubbles and liposomes.

Ying-Zheng Zhao·Yu-Kun Luo·Hai-Dong Liang·Xing-Guo Mei·Jie Tang·Cui-Tao Lu·Yan Zhang·Qian Lin
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Abstract

OBJECTIVE: To compare transfection efficiency and safety for antisense oligodeoxynucleotides (AS-ODNs) between two type of phospholipids-based vectors. METHODS: An AS-ODNs sequence HA824 combined with luciferase reporter plasmid was used. Under low intensity ultrasound (US), a breast cancer cell line SK-BR-3 was exposed to different concentration of microbubbles and liposomes. Transfection efficiency was detected by fluorescence microscopy. Cell viability was verified by propidium iodide assay. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the inhibitory effect of HA824 on HER-2 expression at mRNA level. Atomic force microscopy (AFM) scanning techniques was employed to observe the change of membrane pore size. RESULTS: AS-ODNs transfection efficiency showed an increasing tend with microbubble concentration, but not with liposome concentration. Maximum transfection efficiency with minimum cell viability was achieved under 2% microbubble concentration. Too strong sonoporation activity would enlarge membrane pores significantly and cause low cell viability. CONCLUSION: US-mediated AS-ODNs transfection enhanced by phospholipids-based microbubbles represents an effective and safe avenue.

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Cell Line, TumorCell MembraneCell SurvivalDrug CarriersExcipientsFemaleGene Transfer TechniquesGenes, ReporterGenes, erbB-2HumansLiposomesLuciferasesMicrospheresOligodeoxyribonucleotides, AntisensePhospholipidsPorosityPropidiumRNA, MessengerReverse Transcriptase Polymerase Chain ReactionSpectrophotometry, AtomicTransfectionComparative StudyJournal Article

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Ying-Zheng Zhao, Yu-Kun Luo, Hai-Dong Liang, Xing-Guo Mei, Jie Tang, Cui-Tao Lu, Yan Zhang, Qian Lin. 2006. Comparing transfection efficiency and safety for antisense oligodeoxyribonucleotide between phospholipids-based microbubbles and liposomes.. https://doi.org/10.1080/10611860600965849

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