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Automated Tecan programming for bioanalytical sample preparation with EZTecan.

Huidong Gu·Steve Unger·Yuzhong Deng
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Abstract

This paper reports the first successful automated Tecan (Tecan US, Research Triangle Park, NC) programming for bioanalytical sample analysis with an in-house developed software, called EZTecan. Based on the sample run list in the Watson Laboratory Information Management System (InnaPhase Corp., Philadelphia, PA), EZTecan can generate a Tecan program for bioanalytical sample preparation, including sample transfer, sample dilution, extraction, and reconstitution. Many built-in functions for EZTecan ensure the flexibility, quality, and efficiency of the liquid handling. It takes about 90 min to prepare two 96-well plates from sample transfer to reconstitution. Analytical results shown good inter- and intra-assay accuracy and precision, which meet the +/-15% Good Laboratory Performance acceptance criteria. With EZTecan, bioanalysts can prepare samples without writing a single line of Tecan program.

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Huidong Gu, Steve Unger, Yuzhong Deng. 2006. Automated Tecan programming for bioanalytical sample preparation with EZTecan.. https://doi.org/10.1089/adt.2006.4.721

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