PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 1721036

A new staining method for constriction marks in skin.

W Y Niu·J Z Hu·X M Zhang
View original source ↗Export metadata ↓

Abstract

A modified Poley's acid fuchsin-methyl green stain was used to demonstrate ligature marks in tissues taken at autopsy. This stain was compared in 30 cases with haematoxylin-eosin, Mallory's trichrome and Ogata's picro-indigocarmine stains. Using the modified Poley method, it was possible to demonstrate the compression mark in corpses even when post-mortem changes were advanced. The method was found useful in evaluating the cases when the force used was minimal or when the mark was atypical using the usual haematoxylin-eosin method.

Explore related subjects

False Positive ReactionsForensic MedicineHumansNeckPostmortem ChangesSkinStaining and LabelingWounds and InjuriesJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

W Y Niu, J Z Hu, X M Zhang. 1991. A new staining method for constriction marks in skin.. https://doi.org/10.1016/0379-0738(91)90146-a

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

Novel homogenous time-resolved fluorometric RT-PCR assays for quantification of PSA and hK2 mRNAs in blood.

OBJECTIVES: The purpose of this study was to design, validate, and optimize internally standardized real-time quantitative RT-PCR assays and to identify and avoid problems with assay reliability and examine the impact of an exogenous internal standard. DESIGN AND METHODS: The model system consisted of internally standardized quantitative real-time RT-PCR assays specific for PSA and hK2 mRNA based on time-resolved fluorometric detection of lanthanide chelates. RESULTS: Reproducibility was best when large copy numbers (>5000 per milliliter blood) were analyzed. Addition of an exogenous target-mimicking internal standard had no significant effect on the reproducibility of the method, but increased the calculated copy numbers by an average of 2-fold. CONCLUSIONS: We developed an internally standardized, specific and reproducible real-time RT-PCR analysis method for PSA and hK2 mRNA in circulating cells in the bloodstream. Both PSA and hK2 assays are sufficiently sensitive to detect two LNCaP cells per milliliter whole blood.

False Positive Reactions↗