A red-fluorescent substrate microarray for lipase fingerprinting.

Johann Grognux; Jean-Louis Reymond

doi:10.1039/b609275f

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A red-fluorescent substrate microarray for lipase fingerprinting.

Johann Grognux·Jean-Louis Reymond

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Abstract

A lipase substrate microarray was obtained by printing aliphatic C2-C12 monoesters of (5R)- and (5S)-3-(5,6-dihydroxyhexyloxy)benzaldehyde by reductive alkylation on amine-functionalized glass slides coated with bovine serum albumin and a short PEG linker. The microarray features 12 substrates and their 66 possible binary mixtures spotted in a 9 x 36 spot array. Lipase reactions are detected by chemoselective NaIO(4)-oxidation of the 1,2-diol hydrolysis product to form an aldehyde, which is then tagged with the red-fluorescent dye rhodamine B sulfohydrazide . Specific fingerprints are produced by active enzymes. These experiments provide the first example of lipase fingerprinting using microarrays.

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Cluster Analysis Fluorescent Dyes Lipase Molecular Structure Peptide Mapping Protein Array Analysis Substrate Specificity Evaluation Study Journal Article Research Support, Non-U.S. Gov't

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Johann Grognux, Jean-Louis Reymond. 2006-08-24. A red-fluorescent substrate microarray for lipase fingerprinting.. https://doi.org/10.1039/b609275f

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