Microarray-based kinase inhibition assay by gold nanoparticle probes.

Linlin Sun; Dianjun Liu; Zhenxin Wang

doi:10.1021/ac061687u

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PubMed · 17222050

Microarray-based kinase inhibition assay by gold nanoparticle probes.

Linlin Sun·Dianjun Liu·Zhenxin Wang

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Abstract

We report on the development of a new class of kinase microarray for the detection of kinase inhibition based on marking peptide phosphorylation/biotinylation events by attachment of gold nanoparticles followed by silver deposition for signal enhancement. The alpha-catalytic subunit of cyclic adenosine 5'-monophosphate-dependent protein kinase (PKA), and its well-known substrate, kemptide, were used for the purpose of monitoring phosphorylation and inhibition. As expected, highly selective inhibition of PKA is demonstrated with the four inhibitors: H89, HA1077, mallotoxin, and KN62. Furthermore, an inhibition assay demonstrates the ability to detect kinase inhibition as well as derive IC50 (half-maximal inhibitory concentration) plots.

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Animals Cyclic AMP-Dependent Protein Kinases Gold Humans Inhibitory Concentration 50 Molecular Probes Nanoparticles Oligopeptides Phosphorylation Protein Array Analysis Protein Kinase Inhibitors Protein Kinases Evaluation Study Journal Article Research Support, Non-U.S. Gov't

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Linlin Sun, Dianjun Liu, Zhenxin Wang. 2007-01-15. Microarray-based kinase inhibition assay by gold nanoparticle probes.. https://doi.org/10.1021/ac061687u

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