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Human bcr-abl gene has a lethal effect on embryogenesis.

N Heisterkamp·G Jenster·D Kioussis·P K Pattengale·J Groffen
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Abstract

The chimaeric bcr-abl oncogene is thought to have a crucial role in the development or maintenance of chronic myelogenous leukaemia. To study this oncogene in a more direct way, the bcr-abl gene encoding the P210 protein under control of the bcr gene promoter was introduced into fertilized one-cell embryos, which were then re-implanted into foster mothers. Our data, obtained after several experiments, demonstrate that no live transgenic progeny could be obtained using this bcr-abl construct. The bcr gene is expressed in the course of embryogenesis and the bcr-abl gene product appears to have a pleiotropic lethal effect during this period of development. In concordance, several gross abnormalities were observed while no evidence of neoplastic formation was found. These results suggest that the bcr-abl encoded protein severely affects the process of normal embryogenesis.

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AnimalsBase SequenceBlotting, NorthernEmbryo, MammalianFetal DeathFusion Proteins, bcr-ablGenes, LethalGenes, ablHumansMiceMice, TransgenicMolecular Sequence DataOligodeoxyribonucleotidesOncogenesPolymerase Chain ReactionRNARestriction MappingJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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N Heisterkamp, G Jenster, D Kioussis, P K Pattengale, J Groffen. 1991. Human bcr-abl gene has a lethal effect on embryogenesis.. https://doi.org/10.1007/bf02512996

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