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PubMed · 1749309

Acute sodium-dependent changes in membrane dynamic properties.

K H Le Quan Sang·M Mazeaud·M G Pernollet·D Ben Ishay·M A Devynck
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Abstract

Na+ ions, which can play a pathogenic role in the development of high blood pressure, have been reported to regulate membrane enzymatic activities, receptor-ligand interaction and coupling of G-protein receptors to their effectors. This study was designed to investigate the in vitro effects of Na+ ions on membrane dynamic properties. The fluorescence anisotropy values of TMA-DPH (trimethylamino-diphenylhexatriene, probe selectively incorporated into the outer leaflet of the plasma membrane) was evaluated in platelets and erythrocytes of sodium-dependent hypertension-prone and -resistant rats of the Sabra Strain. Whereas no difference was observed between the 2 strains, TMA-DPH anisotropy was found to be strongly influenced in platelets by external Na+ ions. In the absence of external Na+, TMA-DPH anisotropy increased in human and rat platelets. In contrast, Na+ ions did not affect the anisotropy when the probe was inserted into erythrocyte ghosts. This indicates that Na+ ions can acutely regulate order parameter and microviscosity of platelet plasma membrane in the regions explored by the probe.

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AnimalsBlood PlateletsCell MembraneDiphenylhexatrieneErythrocyte MembraneFluorescence PolarizationMaleMembrane FluidityRatsRats, Inbred StrainsSodiumViscosityJournal Article

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K H Le Quan Sang, M Mazeaud, M G Pernollet, D Ben Ishay, M A Devynck. 1991. Acute sodium-dependent changes in membrane dynamic properties.. https://doi.org/10.1016/0024-3205(91)90639-s

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