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PubMed · 1799657

Platelet-induced granulocyte aggregation in vitro.

T Maeda·G B Nash·B Christopher·Z Pecsvarady·J A Dormandy
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Abstract

Recent studies have shown that the response of granulocytes to stimulation is increased by the presence of platelets. Using an assay based on counting aggregates with a Coulter Counter, we found that heparinized or citrated platelet-rich plasma directly caused aggregation of isolated granulocytes. Platelets themselves were frequently present within the granulocyte aggregates. The degree of aggregation depended on the platelet:granulocyte ratio, and increased over a 2--5 min period of mixing, with a slower increase thereafter. Aggregation was calcium-dependent, increased by stimulation of the platelets with ADP or collagen but not serotonin, and only slightly reduced by a thrombin inhibitor (hirudin, at concentrations up to 0.9 U/ml). A similar adhesive interaction in vivo might be relevant to thrombotic and ischaemic pathology.

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Blood PlateletsCalciumCell AggregationDose-Response Relationship, DrugGranulocytesHumansJournal Article

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BibTeXRIS

T Maeda, G B Nash, B Christopher, Z Pecsvarady, J A Dormandy. 1991. Platelet-induced granulocyte aggregation in vitro.. https://pubmed.ncbi.nlm.nih.gov/1799657/

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