Thrombospondin in ligament, meniscus and intervertebral disc.

R R Miller; C A McDevitt

doi:10.1016/0304-4165(91)90015-9

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Thrombospondin in ligament, meniscus and intervertebral disc.

R R Miller·C A McDevitt

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Abstract

The presence of thrombospondin in extracts of ligament, meniscus and human nucleus pulposus was demonstrated by Western blot analysis using an anti-human thrombospondin antibody. Metabolic labeling of ligament fibroblast and meniscal fibrochondrocyte cultures followed by immunoprecipitation with anti-human thrombospondin revealed a single band corresponding to the molecular weight of thrombospondin when analyzed by SDS-PAGE and fluorography under reduced and non-reduced conditions. Thrombospondin, therefore, is a matrix constituent of ligament, knee joint meniscus and the nucleus pulposus and is synthesized by ligament fibroblasts and meniscal fibrochondrocytes in vitro.

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Animals Ankle Joint Blotting, Western Cartilage, Articular Cattle Dogs Fibroblasts Humans Intervertebral Disc Knee Joint Ligaments, Articular Menisci, Tibial Platelet Membrane Glycoproteins Sheep Thrombospondins Journal Article Research Support, U.S. Gov't, P.H.S.

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R R Miller, C A McDevitt. 1991-11-14. Thrombospondin in ligament, meniscus and intervertebral disc.. https://doi.org/10.1016/0304-4165(91)90015-9

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