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PubMed · 2014543

Intraperitoneal sensitization and the transplanted mouse heart.

G Hisatake·K Bishop·J Shelby·J Sullivan·Y L Shao·E J Eichwald
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Abstract

Intradermal or skin-graft sensitization always results in accelerated rejection of subsequent MHC-disparate mouse heart transplants; intravenous or intrasplenic sensitization almost always results in prolonged survival. The survival time after intraperitoneal sensitization is unpredictable: hyperacute or accelerated rejection, as well as normal or prolonged survival, occurs suggestive of a delicate balance between suppressive and rejecting immune responses. We have observed a positive correlation between transplant survival and the distribution of 51Cr-labeled antigenic cells early after i.v., i.d., i.p., and intrasplenic injection. Label recovery from the viscera was already high 2 hr after i.v. injection, while after i.d. injection virtually all label was still confined to the carcass, even at the end of a 24-hr period. After i.p. injection label recovery varied significantly from mouse to mouse, from very low to a level approximating that seen after i.v. injection. This close correlation suggests that the fate of a subsequent transplant is decided within a few hours after antigen deposition, well before placement of the transplant itself.

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AnimalsGerm-Free LifeGraft RejectionGraft SurvivalHeart TransplantationImmunotherapy, AdoptiveInjections, IntraperitonealMiceMice, Inbred BALB CMice, Inbred StrainsSpleenJournal ArticleResearch Support, Non-U.S. Gov't

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G Hisatake, K Bishop, J Shelby, J Sullivan, Y L Shao, E J Eichwald. 1991. Intraperitoneal sensitization and the transplanted mouse heart.. https://doi.org/10.1097/00007890-199104000-00023

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