Two pathways for GM2(NeuGc) expression in mice: genetic analysis.

M Kono; M Sekine; K Nakamura; Y Hashimoto; Y Seyama; T Yamakawa; A Suzuki

doi:10.1093/oxfordjournals.jbchem.a123333

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Two pathways for GM2(NeuGc) expression in mice: genetic analysis.

M Kono·M Sekine·K Nakamura·Y Hashimoto·Y Seyama·T Yamakawa·A Suzuki

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Abstract

We have reported that WHT/Ht mice express neither GM2(NeuGc) nor GM1(NeuGc) in the liver or erythrocytes due to a defect on the Ggm-2 gene, which was demonstrated to control the activity of UDP-GalNAc:GM3(NeuGc) N-acetylgalactosaminyltransferase in mouse liver, and, in addition, WHT/Ht mice do not express a detectable amount of GM2(NeuGc) but do express GM1(NeuGc) in tissues other than the liver and erythrocytes, such as the spleen, thymus, heart, lung, kidney, and testis [Nakamura et al. (1988) J. Biochem. 103, 201-208]. In order to determine whether the phenotype of WHT/Ht mice exhibiting an undetectable amount of GM2(NeuGc) in these tissues is genetically controlled or not, we analyzed the expression of gangliosides in the progeny obtained on backcross mating between (BALB/c X WHT/Ht)F1 and WHT/Ht mice, and in a GM2(NeuGc) congenic mouse, WHT.C. Concerning the expression of GM2(NeuGc) in the liver, lung, and kidney, 102 backcross mice could be segregated into two types. One type expressed a detectable amount of GM2(NeuGc) in the liver, lung, and kidney, and the other type did not. The ratio of the numbers of mice exhibiting these two types was 42: 60, indicating that the two phenotypes were genetically determined by the involvement of a single autosomal gene. Recombination as to GM2(NeuGc) expression in the liver, lung, and kidney was not detected among the 102 backcross mice. Analysis of the GM2(NeuGc) congenic mouse indicated that a detectable amount of GM2(NeuGc) was expressed in the liver, erythrocytes, lung, kidney, heart, spleen, and small intestine.(ABSTRACT TRUNCATED AT 250 WORDS)

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Animals Brain Crosses, Genetic Female G(M2) Ganglioside Gene Expression Kidney Liver Lung Male Mice Mice, Inbred DBA Mice, Inbred Strains Tissue Distribution Journal Article Research Support, Non-U.S. Gov't

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M Kono, M Sekine, K Nakamura, Y Hashimoto, Y Seyama, T Yamakawa, A Suzuki. 1991. Two pathways for GM2(NeuGc) expression in mice: genetic analysis.. https://doi.org/10.1093/oxfordjournals.jbchem.a123333

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