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PubMed · 2055492

Cis-regulatory elements that confer differential expression upon the rat gene encoding phosphoenolpyruvate carboxykinase in kidney and liver.

T Shoshani·N Benvenisty·M Trus·L Reshef
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Abstract

The PCK gene, encoding cytosolic phosphoenolpyruvate carboxykinase, is specifically expressed in gluconeogenic tissues, liver and kidney. Hence it serves as a model of a class of single-copy genes whose transcription is restricted to a few tissues, rather than a unique tissue. To begin delineating the mechanisms that govern this pattern of expression, cis-regulatory elements of PCK were examined using transient transfection assays in PCK-expressing kidney and hepatoma cell lines. The analyses enabled us to identify a proximal element, between nucleotide (nt) positions -121 and -98, relative to the transcription start point that is sufficient for specific expression in kidney cells, but is just one of the elements required for expression in hepatoma cells. A distal element (between nt -487 and -417), which is essential for hepatoma-specific expression, is not needed in kidney cells. We suggest that the differential regulation of PCK expression in the liver and kidney results from an interplay between different cis-regulatory elements and trans-acting factors.

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AnimalsCCAAT-Enhancer-Binding ProteinsCell LineChromosome DeletionCytosolDNA-Binding ProteinsGene Expression Regulation, EnzymologicKidneyLiverLiver Neoplasms, ExperimentalMutationNFI Transcription FactorsNuclear ProteinsPhosphoenolpyruvate CarboxylasePromoter Regions, GeneticRatsSwineTrans-ActivatorsTranscription FactorsTransfectionTumor Cells, CulturedY-Box-Binding Protein 1Journal ArticleResearch Support, Non-U.S. Gov't

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T Shoshani, N Benvenisty, M Trus, L Reshef. 1991-05-30. Cis-regulatory elements that confer differential expression upon the rat gene encoding phosphoenolpyruvate carboxykinase in kidney and liver.. https://doi.org/10.1016/0378-1119(91)90424-a

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