Multiple GTP-binding proteins from cholinergic synaptic vesicles.

J K Ngsee; K Miller; B Wendland; R H Scheller

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PubMed · 2105379

Multiple GTP-binding proteins from cholinergic synaptic vesicles.

J K Ngsee·K Miller·B Wendland·R H Scheller

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Abstract

Cholinergic synaptic vesicles purified from the electric organ of the marine ray, Discopyge ommata, contain 2 different size classes of GTP-binding proteins: one or more with an apparent molecular weight (MW) between 37 and 41 kDa, and 3 major and at least 2 minor proteins with MW between 20 and 29 kDa. These GTP-binding proteins were detectable using the alpha 32P-GTP overlay technique and covalent modification with bacterial toxins. The higher MW GTP-binding proteins are ADP-ribosylated by pertussis toxin and 2 of the lower MW GTP-binding proteins are sensitive to botulinum toxin.

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Adenosine Diphosphate Ribose Animals Botulinum Toxins Chromatography Fishes GTP-Binding Proteins Molecular Weight Parasympathetic Nervous System Pertussis Toxin Synaptic Vesicles Virulence Factors, Bordetella Journal Article

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J K Ngsee, K Miller, B Wendland, R H Scheller. 1990. Multiple GTP-binding proteins from cholinergic synaptic vesicles.. https://pubmed.ncbi.nlm.nih.gov/2105379/

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