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PubMed · 2273262

The use of tetramethylbenzidine for solid phase immunoassays.

J L McKimm-Breschkin
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Abstract

A rapid, sensitive, solid-phase immunoassay, using horseradish peroxidase and 3,3',5,5'-tetramethylbenzidine, was found to be more sensitive and the colour developed was more stable compared with HRPO using 4-chloro-1-naphthol or diaminobenzidine. Assay sensitivity was equal to or greater than that obtained with the alkaline phosphatase reaction. The essential step was to incubate filters in 1% dextran sulphate for 10 min in a pH 5 (10 mM citrate-EDTA) buffer before reacting with TMB (0.05 mg/ml in the same buffer).

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BenzidinesColorHorseradish PeroxidaseImmunoblottingImmunoenzyme TechniquesSensitivity and SpecificityComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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J L McKimm-Breschkin. 1990-12-31. The use of tetramethylbenzidine for solid phase immunoassays.. https://doi.org/10.1016/0022-1759(90)90282-z

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Electronic, infrared, and 1HNMR spectral studies of the novel charge-transfer complexes of o-tolidine and p-toluidine with alternation pi-acceptors (3,5-dinitro benzoic acid and 2,6-dichloroquinone-4-chloroimide) in CHCl3 solvent.

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