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PubMed · 2317657

Sperm motility, velocity and migration.

D F Badenoch·H D Moore·W V Holt·P R Evans·B S Sidhu·S J Evans
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Abstract

Four different methods for evaluating sperm motility were analysed for experimental error: subjective assessment of a wet film preparation, sperm velocity measured by time-lapse photography, sperm velocity measured by computer analysis and sperm migration across a nucleopore membrane. Subjective assessment of motility was found to be inaccurate, within single observer and between 2 observers. Both methods of measuring mean sperm velocity were accurate, particularly that using the computer analysis system; a high technical failure rate was found using time-lapse photography. Sperm migration across a nucleopore membrane was found to be highly inaccurate. Two groups were then analysed for the predictive value of these tests (excluding sperm migration): 104 proven fertile men and 53 infertile men. Although subjective motility was able to predict from which group the sample came at optimum cut-off with 78% accuracy, computer analysed sperm velocity could predict with overall 91% accuracy at optimum cut-off. Computer analysis of sperm velocity offers a rapid, objective and predictive assessment of sperm function.

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HumansImage Interpretation, Computer-AssistedInfertility, MaleMaleMethodsPhotographyPredictive Value of TestsSperm MotilityVideotape RecordingJournal Article

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D F Badenoch, H D Moore, W V Holt, P R Evans, B S Sidhu, S J Evans. 1990. Sperm motility, velocity and migration.. https://doi.org/10.1111/j.1464-410x.1990.tb14701.x

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