PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 235239

[Quantitative method for determining variamycin].

N B Dzegilenko·V B Korchagin·I V Zhdanovich·Z V Petrakushina
View original source ↗Export metadata ↓

Abstract

A quantitative spectrophotmetric method for the assay of variamycin in commercial samples was developed. It was based on the measurement of the optical density of variamycin solutions in 0.01 N hydrochloric acid at a UV spectrum wave length of 412 nm. A method of thin-layer chromatography for a semi-quantitative estimation of tetraside, the main admixture of variamycin was devised. On storage for 18 months the content of variamycin in the preparations did not change as compared to that at the moment of the drug manufacturing and the amount of tetraside in 18 months did not exceed 2 per cent.

Explore related subjects

Antibiotics, AntineoplasticChromatography, Thin LayerDrug StorageHydrogen-Ion ConcentrationPlicamycinSpectrophotometry, UltravioletTime FactorsJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

N B Dzegilenko, V B Korchagin, I V Zhdanovich, Z V Petrakushina. 1975. [Quantitative method for determining variamycin].. https://pubmed.ncbi.nlm.nih.gov/235239/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1979

Strand scission of superhelical and linear duplex DNAs by the antitumor protein macromomycin. Relationship of in vitro DNA damage to cell growth inhibition.

Macromomycin, a protein antitumor drug, was found to cause strand scissions in vitro in superhelical PM2 and SV40 DNA as well as linear duplex lambda DNA. DNA damage appeared to be single rather than double-strand scissions, and there is an indication that DNA breaks occur at some preferential base sites. The DNA breaks were predominantly true single-strand scissions as opposed to alkali-labile bonds. The cutting reaction was inhibited by low temperature (0 degrees C) and reached a maximum at 45 degrees C. The reaction was not affected by 2-mercaptoethanol, although EDTA did cause a slight decrease in the reaction rate. MgCl2 was found to be an effective inhibitor of the strand scission activity of the drug. The rate of DNA cutting was linear over a wide range of DNA substrate levels. There appeared to be a correlation between the drug's ability to damage DNA and to inhibit cell growth in that similar losses of these two activities occurred as the drug was thermally denatured.

Antibiotics, Antineoplastic↗