PubMed · 2377226
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M J Stark. 1990-08-09. Toxin arrest.. https://doi.org/10.1038/346518b0
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Killer toxin from Saccharomyces cerevisiae SK was isolated by ultrafiltration of culture supernatants and purified by poly(ethylene glycol). The toxin migrates as one single protein band on SDS-PAGE and its molecular weight is 15 kD. The SK toxin has the greatest lethal effect on the sensitive yeast strain in the lat-lag phase. Extraction and purification of killer heretity factor(dsRNA) from SK found that M-dsRNA plasmid and L-dsRNA plasmid have different molecular lengths being 1.7 kb and 4.0 kb.
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A new rapid assay of the yeast killer toxin K1 activity in Saccharomyces cerevisiae, the BCP (bromocresol purple) fluorescence test, was compared with two standard methods, the plating test and the well test. The BCP test is as precise as the plating test and more sensitive than the well test. Under suitable conditions, the test is universally applicable for determining the proportion of cells with a damaged plasma membrane in a population. A procedure was developed for calculating the results of all three tests in terms of a single well-defined unit (lethal or killer unit, LU).