Polymorphonuclear leukocyte phagocytosis: quantitation by a rapid radioactive method.

D S Miller; S Beck

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PubMed · 239077

Polymorphonuclear leukocyte phagocytosis: quantitation by a rapid radioactive method.

D S Miller·S Beck

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Abstract

A rapid quantitative assay of phagocytosis has been developed. The method has been used to determine the phagocytic capability of human or dog polymorphonuclear leukocytes (PMN's) in a defined medium without added sera. The engulfment of 3H-labeled Staphylococcus albus is essentially complete in 30 minutes at 37 degrees C. Increase of phagocyte radioactivity is directly proportional to PMN per cubic centimeter over a wide range of cell concentrations. Maximal phagocytosis is observed at pH 7.5.

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Animals Culture Media Dogs Humans Hydrogen-Ion Concentration Immunologic Techniques In Vitro Techniques Leukocytes Phagocytosis Staphylococcus Time Factors Tritium Journal Article Research Support, U.S. Gov't, P.H.S.

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D S Miller, S Beck. 1975. Polymorphonuclear leukocyte phagocytosis: quantitation by a rapid radioactive method.. https://pubmed.ncbi.nlm.nih.gov/239077/

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