Video-based image collection for quantitative histopathology.

S W Jordan; J M Brayer; P H Bartels; R E Anderson

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PubMed · 2451527

Video-based image collection for quantitative histopathology.

S W Jordan·J M Brayer·P H Bartels·R E Anderson

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Abstract

Obtaining histologic images for computer-based morphometric analysis is associated with a number of standardization problems, which must be solved if reproducible data collection is expected. These problems include tissue processing, sectioning and staining, standardizing and calibrating the video camera and determining the appropriate sampling rate (pixels/micron). Suggested solutions for these problems are presented for a specific image analysis system, but are applicable to other systems with similar capabilities. Biologic variability is not eliminated by computer-assisted analysis, so it is important to minimize data-collection artifacts by parallel processing of experimental and control material, as in other investigative work.

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Animals Histological Techniques Image Processing, Computer-Assisted Kidney Mice Staining and Labeling Video Recording Journal Article Research Support, U.S. Gov't, P.H.S.

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S W Jordan, J M Brayer, P H Bartels, R E Anderson. 1988. Video-based image collection for quantitative histopathology.. https://pubmed.ncbi.nlm.nih.gov/2451527/

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