PubMed · 2472537
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S P Misra, M Dwivedi. 1989-07-08. Hepatitis A vaccine.. https://doi.org/10.1016/s0140-6736(89)90363-2
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When the growth kinetics of immature hepatitis A virus provirions and mature virions were monitored, distinct eclipse phases were noted for both types of particles. Strikingly, uncoating of virions occurred around 4 h postinfection, while uncoating of provirions occurred predominantly between 8 and 10 h postinfection. It is proposed that the heterogeneous mixture of infectious hepatitis A virus particles (virions and provirions) typically present in inocula is responsible for the normally asynchronous nature of hepatitis A virus uncoating kinetics.
BACKGROUND AND OBJECTIVES: A collaborative study was organised to establish a hepatitis A virus (HAV) RNA standard for genomic amplification assays (GAT). MATERIALS AND METHODS: A panel of 10 samples consisting of a 10-fold serial dilution of wild-type HAV diluted in HAV-negative cryosupernatant and samples of the diluent were sent to 14 laboratories in duplicate for testing for HAV RNA by the polymerase chain reaction. RESULTS: Data returned by 12 laboratories indicated that the sensitivities of the assays performed by different laboratories were fairly close: there was a 100-fold difference in sensitivity between the majority of laboratories. Only one laboratory reported false-positive results. CONCLUSION: A 10-5 dilution of the virus in cryosupernatant could be an appropriate working reagent for GAT assays for HAV RNA in plasma pools.
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