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Effective method for obtaining long nucleotide chains on partially complementary templates. Processed bovine gamma-interferon gene obtained from human gamma-interferon gene.

V M Rostapshov·I P Chernov·T L Azhikina·A M Borodin·E D Sverdlov
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Abstract

The method of obtaining the bovine gamma-interferon gene by means of simultaneous multidirected mutagenesis of the human gamma-interferon gene is presented. The first strand of the bovine gamma-interferon gene was obtained by ligation of synthetic oligonucleotides, using the cDNA of human gamma-interferon, cloned in the single-stranded phage M13mp19 as a template. The second strand was synthesized using a large fragment of E. coli DNA-polymerase I. The double-stranded gene was then treated by restriction nucleases and cloned in a pUC-18 derived vector. The primary structure was confirmed by sequencing.

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AnimalsBase SequenceCattleElectrophoresis, Agar GelHumansInterferon-gammaMolecular Sequence DataOligonucleotidesSequence Homology, Nucleic AcidTemplates, GeneticJournal Article

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V M Rostapshov, I P Chernov, T L Azhikina, A M Borodin, E D Sverdlov. 1989-06-05. Effective method for obtaining long nucleotide chains on partially complementary templates. Processed bovine gamma-interferon gene obtained from human gamma-interferon gene.. https://doi.org/10.1016/0014-5793(89)80663-5

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