Protein engineering and design.

T L Blundell; G Elliott; S P Gardner; T Hubbard; S Islam; M Johnson; D Mantafounis; P Murray-Rust; J Overington; J E Pitts

doi:10.1098/rstb.1989.0059

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Protein engineering and design.

T L Blundell·G Elliott·S P Gardner·T Hubbard·S Islam·M Johnson·D Mantafounis·P Murray-Rust·J Overington·J E Pitts

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Abstract

Rapid advances in site-directed mutagenesis and total gene synthesis combined with new expression systems in prokaryotic and eukaryotic cells have provided the molecular biologist with tools for modification of existing proteins to improve catalytic activity, stability and selectivity, for construction of chimeric molecules and for synthesis of completely novel molecules that may be endowed with some useful activity. Such protein engineering can be seen as a cycle in which the structures of engineered molecules are studied by X-ray analysis and two-dimensional nuclear magnetic resonance. The results are used in the improvement of the design by using knowledge-based procedures that exploit facts, rules and observations about proteins of known three-dimensional structure.

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Amino Acid Sequence Animals Base Sequence Crystallins Genes Genetic Engineering Humans Models, Molecular Molecular Sequence Data Mutation Protein Conformation Proteins Recombinant Proteins Sequence Homology, Nucleic Acid Journal Article Review

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T L Blundell, G Elliott, S P Gardner, T Hubbard, S Islam, M Johnson, D Mantafounis, P Murray-Rust, J Overington, J E Pitts. 1989-08-31. Protein engineering and design.. https://doi.org/10.1098/rstb.1989.0059

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