A catalytic 13-mer ribozyme.

A C Jeffries; R H Symons

doi:10.1093/nar/17.4.1371

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A catalytic 13-mer ribozyme.

A C Jeffries·R H Symons

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Abstract

A 13-mer oligoribonucleotide can act as a ribozyme for the specific self-cleavage of a 41-mer oligoribonucleotide substrate in the presence of Mg2+. The two sequences involved correspond to the self-cleavage hammerhead structure of the virusoid of lucerne transient streak virus. The Michaelis-menten kinetic parameters for the reaction were; Km 1.3 microM, Vmax 0.012 microM min-1, kcat 0.5 min-1. The 13-mer RNA is the smallest ribozyme so far reported. A DNA analogue of the 13-mer can not substitute for the RNA in the reaction.

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Animals Base Sequence Fishes Kinetics Molecular Sequence Data Nucleic Acid Conformation Oligodeoxyribonucleotides RNA Precursors RNA Splicing RNA, Catalytic RNA, Ribosomal Journal Article Research Support, Non-U.S. Gov't

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A C Jeffries, R H Symons. 1989-02-25. A catalytic 13-mer ribozyme.. https://doi.org/10.1093/nar%2F17.4.1371

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