Interaction between cortisol and microbial proteases.

W N Kuo; U Ganesan; M N Jean; T B Richardson; A Robinson; A Williams; N Stone; M Young; J Noone; E J Burch

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PubMed · 2699728

Interaction between cortisol and microbial proteases.

W N Kuo·U Ganesan·M N Jean·T B Richardson·A Robinson·A Williams·N Stone·M Young·J Noone·E J Burch

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Abstract

Binding (or interaction) of cortisol with microbial molecule(s) was observed by employing Bio-Gel HTP affinity chromatography and subsequently by fluorescence spectrophotometry. Molecule(s) in the crude extract of baker's yeast and in other microbial proteases exhibited varied degrees of cortisol-binding. Bacterial protease (type IX) had highest, while the type XXVI enzyme had the lowest, binding capacity. In addition, these two proteases exhibited a distinct difference in the alterations of ultraviolet spectra due to interaction with cortisol. Using casein as a substrate, cortisol, CTP, trypsin inhibitor or leupeptin appreciably inhibited type IX protease at low concentrations of Ca2+. However, thyroxine had no effect on this protease.

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Bacteria Endopeptidases Enzyme Inhibitors Fungi Hydrocortisone In Vitro Techniques Journal Article Research Support, U.S. Gov't, P.H.S.

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W N Kuo, U Ganesan, M N Jean, T B Richardson, A Robinson, A Williams, N Stone, M Young, J Noone, E J Burch. 1989. Interaction between cortisol and microbial proteases.. https://pubmed.ncbi.nlm.nih.gov/2699728/

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