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PubMed · 2706139

Interactive morphometry: the influence of magnification.

T Torkkeli·Y Collan
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Abstract

The influence of magnification on light microscopic morphometry was studied. Morphometry was performed on 34 hepatocytes by using a digitizer plate with micrographs of the nuclei, and with digitized image of the nuclei on a separate monitor. Intraobserver variation was smallest at objective magnifications of 40 and 100 X (CV of form factor, maximum diameter and nuclear perimeter under 1%, of nuclear area under 2%, when measured from the monitor). The results show that more reproducible measurements are possible with higher magnifications. The authors recommend objective magnifications of 40 and 100 X for morphometric measurements on nuclei. The results also suggest that morphometric grading should apply an intermediate third grade in dichotomous grading scales.

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AnimalsCell NucleusHistologyImage Processing, Computer-AssistedLiverMicroscopyPathologyRatsJournal Article

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T Torkkeli, Y Collan. 1989. Interactive morphometry: the influence of magnification.. https://pubmed.ncbi.nlm.nih.gov/2706139/

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