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Decrease in CuZn-superoxide dismutase mRNA level during differentiation of human monocytic and promyelotic leukemia cells.

H Saito·T Kuroki·K Nose
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Abstract

Change in the level of CuZn-superoxide dismutase (SOD) mRNA was examined using a molecular probe during differentiation of human monocytic leukemia U937 cells or promyelotic leukemia HL-60 cells induced by either 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or dimethylsulfoxide (DMSO). CuZn-SOD mRNA levels were found to decrease during the course of differentiation, and this response is specific for differentiation, since the treatment of human B cell leukemia cells or normal diploid fibroblasts with TPA failed to have any effect on the level of CuZn-SOD mRNA. The activity of CuZn-SOD in U937 cells also decreased during differentiation, but following that of the CuZn-SOD mRNA level. The expression of the CuZn-SOD gene is thus concluded to diminish during the differentiation of HL-60 and U937 cells.

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Blotting, NorthernCell DifferentiationCopperDimethyl SulfoxideFibroblastsHumansLeukemia, Monocytic, AcuteLeukemia, Promyelocytic, AcuteNucleic Acid HybridizationRNA, MessengerSuperoxide DismutaseTetradecanoylphorbol AcetateTumor Cells, CulturedZincJournal ArticleResearch Support, Non-U.S. Gov't

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H Saito, T Kuroki, K Nose. 1989-06-05. Decrease in CuZn-superoxide dismutase mRNA level during differentiation of human monocytic and promyelotic leukemia cells.. https://doi.org/10.1016/0014-5793(89)80634-9

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