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Compaction-defective embryonal carcinoma cell variants.

J W Littlefield
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Abstract

Compaction of the morula is a prerequisite for subsequent differentiation of the mouse embryo. Analogous differentiation follows compaction in aggregates of several embryonal carcinoma cell lines. This report describes the isolation of two compaction-defective variants from the H6 embryonal carcinoma cell line. These were isolated directly as clonal compaction-defective aggregates in medium containing 1.3% methylcellulose. They were obtained following chemical mutagenesis, since spontaneous variants were not seen. Compaction-defective variants of the F9 ECC line or the ES-D3 embryonic stem cell line could not be obtained. One of the H6 compaction-defective variants appeared to be dominant when hybridized to its parental line, while the other appeared to be recessive.

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AnimalsCalcium ChlorideCell AggregationCell DifferentiationEmbryonal Carcinoma Stem CellsHistocytochemistryHybrid CellsMethylcelluloseMiceNeoplastic Stem CellsTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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J W Littlefield. 1989. Compaction-defective embryonal carcinoma cell variants.. https://doi.org/10.1002/dvg.1020100403

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