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Utilization of an Epstein-Barr virus replicon as a eukaryotic expression vector.

J M Young·C Cheadle·J S Foulke·W N Drohan·N Sarver
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Abstract

Epstein-Barr virus (EBV) replicons which include the genetic element oriP and a functional gene for Epstein-Barr nuclear antigen (EBNA-1) can be maintained episomally in a variety of mammalian cell lines [Yates et al., Nature 313 (1985) 812-815]. We have assessed the application of an EBV replicon for foreign gene expression. Two cDNAs, human interferon-gamma (IFN-gamma) and the extracellular domain of the human epidermal growth factor receptor (EGF-Rex), cloned in an EBV replicon, were efficiently expressed and the protein was secreted into the extracellular media. Expression in human embryonic 293 cells was approximately ten-fold higher than in CV-1 cells. The expression of the human protein is dependent upon the orientation of the IFN-gamma transcriptional cassette relative to the other genetic elements within the vector.

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AnimalsAntigens, ViralCell LineCellsChlorocebus aethiopsCloning, MolecularDNA, RecombinantEmbryo, MammalianEpstein-Barr Virus Nuclear AntigensErbB ReceptorsEukaryotic CellsGenetic VectorsHerpesvirus 4, HumanHumansInterferon-gammaKidneyPlasmidsProtein Processing, Post-TranslationalRNA, MessengerRecombinant Fusion ProteinsRepliconJournal Article

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J M Young, C Cheadle, J S Foulke, W N Drohan, N Sarver. 1988. Utilization of an Epstein-Barr virus replicon as a eukaryotic expression vector.. https://doi.org/10.1016/0378-1119(88)90556-2

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