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Enzyme electrodes and their application.

F Scheller·D Kirstein·L Kirstein·F Schubert·U Wollenberger·B Olsson·L Gorton·G Johansson
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Abstract

Starting from the state of the art, principles for improving the analytical characteristics of enzyme electrodes are discussed. Coupling of appropriate amperometric electrode processes with enzyme systems, e.g. urease or aminopeptidases, results in a simplification of operation. Optimal sample frequencies are realized on the basis of enzyme membranes, with both a small characteristic diffusion time and a high enzyme activity, applied in a well-designed sample-processing system. Coupled enzyme reactions of the sequence or competition type are successfully used for extension to new analytes, e.g. inhibitors, cofactors or alternative substrates. Cyclization of the analyte enhances the sensitivity of enzyme electrodes to the nanomolar concentration range. Enzymic anti-interference layers are a tool for improving the sensor specificity. The operational characteristics of enzyme electrodes are thus adaptable to any given analytical problem.

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BiotechnologyElectrodesEnzymes, ImmobilizedKineticsMicrochemistryJournal Article

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F Scheller, D Kirstein, L Kirstein, F Schubert, U Wollenberger, B Olsson, L Gorton, G Johansson. 1987-08-28. Enzyme electrodes and their application.. https://doi.org/10.1098/rstb.1987.0019

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