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Isolation of a cDNA clone encoding a biologically active thyroid hormone receptor.

R J Koenig·R L Warne·G A Brent·J W Harney·P R Larsen·D D Moore
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Abstract

We have isolated a c-erbA cDNA clone from a GH3 cell library. The clone, denoted erb62, is 4.5 kilobases long and encodes a 461-amino acid beta-type c-erbA protein. This c-erbA protein binds 3,5,3'-triiodothyronine (T3) and T3 analogs with affinities similar to those of the authentic T3 receptor. By RNA gel blot analysis, erb62 hybridizes to a 6-kilobase RNA found in organs that express T3 receptors--e.g., heart, kidney, and brain. A COS-cell transient cotransfection system was used to show that erb62 encodes a biologically active T3 receptor. An oligonucleotide, corresponding to a portion of the rat growth hormone gene 5'-flanking region that contains a T3 response element, was inserted on the 5' side of the herpes simplex virus thymidine kinase promoter in a chloramphenicol acetyltransferase-expressing plasmid. Reporter gene expression directed by this hybrid promoter was T3 inducible only if this plasmid was cotransfected with an erb62-expressing plasmid.

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Amino Acid SequenceAnimalsBase SequenceBrainCell LineDNAElectrophoresis, Polyacrylamide GelGene Expression RegulationKidneyMolecular Sequence DataMolecular WeightMyocardiumNucleic Acid HybridizationPromoter Regions, GeneticProto-Oncogene ProteinsRNA, MessengerRatsReceptors, Thyroid HormoneTransfectionTriiodothyronineJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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R J Koenig, R L Warne, G A Brent, J W Harney, P R Larsen, D D Moore. 1988. Isolation of a cDNA clone encoding a biologically active thyroid hormone receptor.. https://doi.org/10.1073/pnas.85.14.5031

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