Molecular sorting in the secretory pathway.

K N Chung; P Walter; G W Aponte; H P Moore

doi:10.1126/science.2911732

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PubMed · 2911732

Molecular sorting in the secretory pathway.

K N Chung·P Walter·G W Aponte·H P Moore

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Abstract

Proteins can be secreted from animal cells by either a constitutive or a regulated pathway; those destined for regulated secretion are actively sorted into dense-core secretory granules. Although sorting is generally assumed to be accomplished by specific carriers, the nature of these carriers remains elusive. In this study, peptide hormones were used as affinity ligands to purify a set of 25-kilodalton proteins from canine pancreatic tissue. Their ligand specificities and patterns of expression have the characteristics of sorting carriers.

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Animals Antigen-Antibody Complex Carrier Proteins Cell Line Chromatography, Affinity Dogs Golgi Apparatus Immune Sera Immunohistochemistry Intracellular Membranes Molecular Weight Pancreas Prolactin Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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K N Chung, P Walter, G W Aponte, H P Moore. 1989-01-13. Molecular sorting in the secretory pathway.. https://doi.org/10.1126/science.2911732

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