HLA-DR genotyping by restriction fragment length polymorphism analyses.

K Hui; H Festenstein; A de Klein; G Grosveld; F Grosveld

doi:10.1007/bf00404482

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HLA-DR genotyping by restriction fragment length polymorphism analyses.

K Hui·H Festenstein·A de Klein·G Grosveld·F Grosveld

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Abstract

We have established unique restriction fragment length polymorphism (RFLP) patterns characteristic of homozygous typing cells (HTCs) for HLA-DR-1 through HLA-DR-8 haplotypes. These RFLP patterns were found to segregate in family members and correlate 100% with HLA-DR antibody phenotyping. The RFLP patterns were used to type chronic myelocytic leukemic cells which have a Philadelphia translocation from 23 randomly selected Caucasoid patients. The results show an alternative method for the determination of the HLA-DR types without using live cells and to study disease association with the HLA-DR region.

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Cell Line DNA Restriction Enzymes Genotype HLA-DR Antigens Histocompatibility Antigens Class II Humans Nucleic Acid Hybridization Pedigree Philadelphia Chromosome Polymorphism, Genetic Journal Article Research Support, Non-U.S. Gov't

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K Hui, H Festenstein, A de Klein, G Grosveld, F Grosveld. 1985. HLA-DR genotyping by restriction fragment length polymorphism analyses.. https://doi.org/10.1007/bf00404482

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