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Reduced rhodopsin phosphorylation during retinal dystrophy.

D J Takemoto·J Cunnick·L J Takemoto
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Abstract

During inherited retinal dystrophy in Irish Setter dogs, decreased activity of cGMP phosphodiesterase (PDE) results in high cGMP levels and retinal degeneration (1-3). This defect could be in PDE itself, or in its interactions with other proteins of the rod outer segment. We report herein that when retinas from 8-week-old dogs were phosphorylated with gamma-32P-ATP, and separated on SDS-PAGE, phosphorylation of rd dog rhodopsin was reduced. When rd retinas were mixed with normal dog retinas, phosphorylation of the latter was inhibited. Since rd-mediated inhibition was prevented by 1 mM NaF, the results suggest that the cause of reduced rd phosphorylation is increased phosphatase activity. Together, these results demonstrate that decreased phosphorylation of rhodopsin due to increased phosphatase activity is a fundamental biochemical change which may partially account for the degenerative process and loss of visual acuity during inherited retinal dystrophy.

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AnimalsDog DiseasesDogsPhosphoprotein PhosphatasesPhosphorylationRetinaRetinal DegenerationRetinal PigmentsRhodopsinJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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D J Takemoto, J Cunnick, L J Takemoto. 1986-03-28. Reduced rhodopsin phosphorylation during retinal dystrophy.. https://doi.org/10.1016/0006-291x(86)91030-2

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