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PubMed · 3010695

Postembedding immunogold labeling for electron microscopy using "LR White" resin.

B G Timms
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Abstract

A method is described for performing postembedding immunogold immunocytochemistry on sections of LR White-embedded tissues. Fixation of tissue in a combination of paraformaldehyde and glutaraldehyde, or with low concentrations of glutaraldehyde followed by partial dehydration, resulted in preservation of antigenicity for a variety of proteins in different tissue samples. Good structural preservation facilitated high-resolution immunolabeling when coupled with the use of purified monoclonal antibodies. The technique is straightforward and versatile, offering the potential for many immunocytochemical applications with minimal modifications.

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AnimalsAnion Exchange Protein 1, ErythrocyteAntibodies, MonoclonalCa(2+) Mg(2+)-ATPaseCalcium-Transporting ATPasesErythrocytesFemaleGoldHumansImmunochemistryLeukemia, Myeloid, AcuteMicroscopy, ElectronMusclesPeroxidaseRabbitsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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B G Timms. Postembedding immunogold labeling for electron microscopy using "LR White" resin.. https://doi.org/10.1002/aja.1001750211

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