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PubMed · 3011149

T cell-derived migration-inhibitory factor and colony-stimulating factor share common structural elements.

T Kawaguchi·D W Golde·A Mednis·N Bersch·S Clark·H G Remold
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Abstract

Migration-inhibitory factor (MIF) is a lymphokine that acts to localize mononuclear phagocytes (monocytes and macrophages) and perhaps to activate them. Mo cells are a human T cell leukemia virus II-infected T cell line previously shown to secrete large quantities of MIF upon stimulation with phytohemagglutinin and phorbol myristate acetate. MIF was purified from Mo cell-conditioned medium by gel filtration, phenyl-Sepharose affinity chromatography, isoelectrofocusing, and reverse-phase high-performance liquid chromatography (RP-HPLC). Overall purification was 6,000-fold. The purified MIF fraction was found to display potent colony-stimulating factor (CSF) activity when assayed on human bone marrow cells. The double peak of MIF activity as shown by C 18-RP-HPLC coincided with the double peak of CSF activity. A monoclonal antibody selected for its anti-MIF activity absorbed both the CSF and the MIF activity. These findings indicate that MIF and CSF are either identical molecules or closely related molecules with common structural elements.

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Cell LineChromatography, AffinityChromatography, GelChromatography, High Pressure LiquidColony-Stimulating FactorsDeltaretrovirusFluorometryHumansIsoelectric FocusingLymphokinesPhytohemagglutininsTetradecanoylphorbol AcetateComparative StudyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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T Kawaguchi, D W Golde, A Mednis, N Bersch, S Clark, H G Remold. 1986. T cell-derived migration-inhibitory factor and colony-stimulating factor share common structural elements.. https://pubmed.ncbi.nlm.nih.gov/3011149/

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